Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili

The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency food transformation and growth, therefore aquaculture profitability. aim present was assess activity functional characteristics key enzymes in juveniles greater amberjack ( Seriola dumerili ), as well possible modulation their relative importance by water temperature. For that, a combination biochemical assays substrate-SDS-PAGE were used. Under physiological conditions pepsin negligible. Chymotrypsin most active enzyme tract amberjack, while lipase with lower activity, though both addition trypsin responsive temperature revealed discriminant analysis. showed have pH-sensitive and, except for chymotrypsin, thermally robust proteases. Inhibition major serine proteases inverse chymotrypsin responses environmental temperature, higher contribution 26°C-fish 18°C-fish. Zymograms three isotrypsin isochymotrypsin enzymes, no variation presence particular isoforms among rearing temperatures. However, they confirmed role providing plasticity, one being more at Thus, results indicate isoenzymes occurs due different physic-chemical features source flexibility. This assessed first time effects on increasing knowledge its biochemistry, aiding improvement management practices this species industrialization.